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Frokjaer S, Otzen DE

All catheters were flushed with phosphate-buffered saline (0.1 M, pH 7.4) approximately four hours before commencement of the experiments

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If folate deficiency is suspected, it is reasonable to give oral folic acid (0.4 1 mg/day) without doing laboratory investigation for deficiency at least until the hemoglobin and mean corpuscular volume normalizes (or longer if the underlying cause cannot be eliminated)